About 20 years ago Paul Smits and Jacques Lenders introduced the forearm model in our laboratory. The brachial artery is cannulated to infuse adenosine or other drugs of interest. Forearm blood flow is measured using mercury-in-silastic strain-gauge plethysmography.
The hand circulation is occluded to exclude skin circulation as much as possible from the experimental preparation. Blood flow is measured at both forearms simultaneously to detect systemic actions and to control for non-specific random changes in flow that occur for example due to mental arousal or inactivity.
Forearm blood flow is calculated from the velocity of change in forearm volume that occurs during rapid inflations of the upper arm cuffs at supravenous pressures.