Microarrays:
Microarrayspotter, Nanoplotter 2.0 (GeSIM)
The Nano-Plotter is used for the generation of microarrays. However, application of the nanodispensing for example for the functionalization of microstructures is also possible. The system operates by non-contact dispensing of sub-nanoliters volumes. The “drop on demand” piezo technology allows the placement of very small drops onto various surfaces. The spotted volume is adjusted by changing the drop count and by two different kinds of piezoelectric pipetting tips. The Nano-Tip dispenses droplets in a volume of 0.4 nl, the Pico-Tip in a volume of 0.1 nl. A thorough rinsing step between pipetting cycles washes the piezoelectric tips and thus prevents cross contamination of different samples. Spotting parameters can be adjusted for example for pipetting of protein solutions of different concentrations. The system has a temperature-controlled slide support and is contained in a humidified housing, thereby allowing control of the drying process.
The Nano-Plotter work station
Microarray reader (ProScanArray, Perkin Elmer)
This system scans microarrays in the format of 25 x 75.6 x 1 mm. Our Pro ScanArray system includes two lasers (546 nm and 633 nm, available filters: 570 nm, 578 nm, 592 nm, 660 nm, 670 nm, 694 nm) and a workstation running the scanner software. The software is used to control all aspects of scanning, quantitation and saving of the scanned image.
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Image of the microarray scanner Pro ScanArray
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Microarray analysis software (ArrayProAnalyzer, Media Cybernetics)